{"id":461,"date":"2022-03-03T21:04:32","date_gmt":"2022-03-03T13:04:32","guid":{"rendered":"https:\/\/cabit.top\/?p=461"},"modified":"2022-03-28T09:51:08","modified_gmt":"2022-03-28T01:51:08","slug":"flowjo%e5%8a%9f%e8%83%bd%e5%8c%ba%e4%bb%8b%e7%bb%8d","status":"publish","type":"post","link":"https:\/\/cabit.top\/?p=461","title":{"rendered":"FlowJo\u529f\u80fd\u533a\u4ecb\u7ecd"},"content":{"rendered":"\n

FlowJo\u5de5\u4f5c\u533a\u53ef\u4ee5\u4f7f\u7528\u529f\u80fd\u533a\u4e3a\u7ecf\u5e38\u4f7f\u7528\u7684\u64cd\u4f5c\u8fdb\u884c\u81ea\u5b9a\u4e49\u3002<\/p>\n\n\n\n

\u529f\u80fd\u533a\u7684\u57fa\u672c\u7ed3\u6784\u662f\u6807\u7b7e<\/strong>\uff08<\/strong>tabs<\/strong>\uff09<\/strong>\uff0c\u6bcf\u4e2a\u6807\u7b7e<\/strong>\u5185\u6709\u591a\u4e2a\u533a<\/strong>\uff08<\/strong>bands<\/strong>\uff09<\/strong>\u3002\u56fe 1 \u663e\u793a\u4e86 FlowJo \u5de5\u4f5c\u533a\u53ca\u5176\u529f\u80fd\u533a\uff0c\u8be5\u529f\u80fd\u533a\u5c06\u529f\u80fd\u7ec4\u7ec7\u5230\u4e00\u7cfb\u5217\u9009\u9879\u5361\u4e2d\uff0c\u4fbf\u4e8e\u8f7b\u677e\u5bfc\u822a\u3002<\/p>\n\n\n\n

\"\"
\u56fe 1. FlowJo \u5de5\u4f5c\u533a\u529f\u80fd\u533a<\/strong><\/figcaption><\/figure>\n\n\n\n

\u5e94\u7528\u7a0b\u5e8f\u6309\u94ae\uff08Application Button\uff09\u5305\u542b\u4fdd\u5b58\/\u6253\u5f00\u548c\u5de5\u4f5c\u533a\u521b\u5efa\u529f\u80fd\u3002\u4e00\u4e2a\u9009\u9879\u5361\u5305\u542b\u591a\u4e2a\u6309\u4e0a\u4e0b\u6587\u7ec4\u7ec7\u7684\u533a\uff08bands\uff09\u3002\u4efb\u52a1\u680f\u63d0\u4f9b\u5bf9\u5e38\u7528\u5de5\u5177\u7684\u5feb\u901f\u8bbf\u95ee\u3002\u529f\u80fd\u533a\u652f\u6301\u4ee5\u4e0b\u64cd\u4f5c\uff1a<\/p>\n\n\n\n

1. <\/strong>\u6536\u7f29\/\u6269\u5c55<\/strong>\uff1a\u5982\u679c\u60a8\u8c03\u6574\u7a97\u53e3\u5927\u5c0f\uff0c\u60a8\u4f1a\u6ce8\u610f\u5230\u533a\uff08band\uff09\u7684\u4e0d\u540c\u7ec4\u4ef6\u6536\u7f29\u548c\u6269\u5c55\u3002\u8bf7\u6ce8\u610f\uff0c\u67d0\u4e9b\u529f\u80fd\u4ec5\u5728\u529f\u80fd\u533a\u5b8c\u5168\u5c55\u5f00\u65f6\u53ef\u7528\u3002\u529f\u80fd\u533a\u4e0a\u7684\u533a\u5728\u5176\u53f3\u4fa7\u7684\u533a\u4e4b\u524d\u5c55\u5f00\u3002<\/p>\n\n\n\n

2. <\/strong>\u9690\u85cf\/\u663e\u793a<\/strong>\uff1a\u5982\u679c\u53cc\u51fb\u529f\u80fd\u533a\u4e2d\u7684\u4efb\u4f55\u9009\u9879\u5361\uff0c\u529f\u80fd\u533a\u5c06\u6298\u53e0\u3002\u518d\u6b21\u53cc\u51fb\u9009\u9879\u5361 – \u529f\u80fd\u533a\u5c06\u5c55\u5f00\u3002\u8fd9\u53ef\u4ee5\u5728\u7b14\u8bb0\u672c\u7535\u8111\u663e\u793a\u5668\u4e0a\u8282\u7701\u5b9d\u8d35\u7684\u5782\u76f4\u50cf\u7d20\u3002<\/p>\n\n\n\n

3. <\/strong>\u6eda\u8f6e<\/strong> \u652f\u6301<\/strong>\uff1a\u5982\u679c\u60a8\u7684\u9f20\u6807\u6709\u6eda\u8f6e\uff0c\u60a8\u53ef\u4ee5\u4f7f\u7528\u5b83\u6765\u6d4f\u89c8\u9009\u9879\u5361\u3002<\/p>\n\n\n\n

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5. <\/strong>\u79fb\u9664\u533a<\/strong>\uff1a\u533a\u5f88\u5bb9\u6613\u4ece\u529f\u80fd\u533a\u4e0a\u53bb\u9664\u3002\u9996\u5148\uff0c\u5c55\u5f00\u7a97\u53e3\uff0c\u4f7f\u533a\u5b8c\u5168\u5448\u73b0\u3002\u7136\u540e\u53ea\u9700\u5c06\u4e0d\u9700\u8981\u7684\u533a\u4ece\u7236\u7a97\u53e3\u4e2d\u62d6\u51fa – \u5f53\u60a8\u677e\u5f00\u9f20\u6807\u65f6\uff0c\u5b83\u4eec\u5c31\u4f1a\u6d88\u5931\u3002<\/p>\n\n\n\n

6. <\/strong>\u5e2e\u52a9<\/strong>\uff1a\u53f3\u952e\u5355\u51fb\u4e00\u4e2a\u533a\u5c06\u663e\u793a\u4e00\u4e2a\u6307\u5411\u6b64\u6587\u6863\u7ad9\u70b9\u7684\u5e2e\u52a9\u94fe\u63a5\u3002\u5c06\u9f20\u6807\u60ac\u505c\u5728\u533a\u4e2d\u7684\u67d0\u4e2a\u9879\u76ee\u4e0a\u5c06\u663e\u793a\u4e00\u4e2a\u4e30\u5bcc\u7684\u5de5\u5177\u63d0\u793a\uff0c\u5176\u4e2d\u63cf\u8ff0\u4e86\u8be5\u5de5\u5177\u7684\u529f\u80fd\u548c\u952e\u76d8\u5feb\u6377\u952e\u3002<\/p>\n\n\n\n

\u4e0b\u9762\u5217\u51fa\u7684 FlowJo\u533a\uff1a<\/strong><\/p>\n\n\n\n

1)<\/strong> \u5de5\u4f5c\u533a\u529f\u80fd\u533a\uff1a Workspace\uff08\u5de5\u4f5c\u533a\uff09 \u662f\u5206\u6790\u7684\u4e2d\u5fc3\u67a2\u7ebd\uff0c\u56e0\u6b64\u5176\u529f\u80fd\u533a\u662f\u6700\u590d\u6742\u7684\u3002\u9ed8\u8ba4\u60c5\u51b5\u4e0b\uff0c\u53ea\u6709\u6240\u6709\u53ef\u80fd\u533a\u7684\uff08bands\uff09\u4e00\u4e2a\u5b50\u96c6\u5b89\u88c5\u5728\u529f\u80fd\u533a\u4e2d\u3002<\/p>\n\n\n\n

\"\"
\u56fe 1.1\uff1a\u5de5\u4f5c\u533a\u529f\u80fd\u533a<\/strong><\/figcaption><\/figure>\n\n\n\n

2)<\/strong>\u00a0\u5e03\u5c40\u7f16\u8f91\u5668\u529f\u80fd\u533a\uff1a\u5e03\u5c40\u7f16\u8f91\u5668\u7528\u4e8e\u4ee5\u56fe\u5f62\u65b9\u5f0f\u5448\u73b0\u60a8\u7684\u5206\u6790\uff0c\u56e0\u6b64\u6b64\u529f\u80fd\u533a\u7684\u91cd\u70b9\u662f\u62a5\u544a\u751f\u6210\u3001\u5bf9\u9f50\u3001\u56fe\u5f62\u548c\u6253\u5370\u3002v10\u00a0\u4e2d\u6709\u8bb8\u591a\u65b0\u7684\u6837\u5f0f\u529f\u80fd\uff0c\u53ef\u4ee5\u5728\u201cObject(\u5bf9\u8c61)\u201d\u548c\u201cArrange(\u6392\u5217)\u201d\u9009\u9879\u5361\u4e2d\u627e\u5230\u3002<\/p>\n\n\n\n

\"\"
\u56fe\u00a02\uff1a\u5e03\u5c40\u7f16\u8f91\u5668\u529f\u80fd\u533a\u3002<\/strong><\/figcaption><\/figure>\n\n\n\n

3)<\/strong>\u00a0\u8868\u683c\u7f16\u8f91\u5668\u529f\u80fd\u533a\uff1a\u00a0\u8868\u683c\u7f16\u8f91\u5668\u00a0\u7528\u4e8e\u4ee5\u6570\u5b57\u65b9\u5f0f\u5448\u73b0\u60a8\u7684\u5206\u6790\uff0c\u56e0\u6b64\u53ef\u7528\u7684\u5de5\u5177\/\u64cd\u4f5c\u7528\u4e8e\u6279\u5904\u7406\u9009\u9879\u548c\u5b9a\u4e49\u8868\u683c\u4e2d\u7684\u5217\u3002\u6b64\u7a97\u53e3\u652f\u6301\u70ed\u56fe\u548c\u57fa\u4e8e\u533a\u57df\u7684\u683c\u5f0f\uff0c\u8fd8\u5c06\u521b\u5efa\u56fe\u8868\u4ee5\u663e\u793a\u65f6\u95f4\u5e8f\u5217\u3001\u76f8\u5173\u6027\u3001\u7ebf\u56fe\u6216\u6570\u636e\u7684\u00a03D\u00a0\u89c6\u56fe\u3002<\/p>\n\n\n\n

\"\"
\u56fe\u00a03\uff1a\u8868\u7f16\u8f91\u5668\u529f\u80fd\u533a<\/strong><\/figcaption><\/figure>\n\n\n\n

4)<\/strong>\u00a0\u677f\u7f16\u8f91\u5668\u529f\u80fd\u533a\uff1a\u677f\u7f16\u8f91\u5668\u53ef\u4ee5\u4ece\u57fa\u4e8e\u677f\u7684\u5206\u6790\u4e2d\u6ce8\u91ca\u00a0FCS\u00a0\u6587\u4ef6\u00a0\u3002\u8bb8\u591a\u7528\u6237\u4fdd\u7559\u4ed6\u4eec\u7684\u6837\u54c1\u548c\u6837\u54c1\u4fe1\u606f\u7684\u00a0Excel\u00a0\u7535\u5b50\u8868\u683c\uff0c\u4ee5\u5e2e\u52a9\u7ec4\u7ec7\u4ed6\u4eec\u7684\u6570\u636e\u6587\u4ef6\u5e76\u8ddf\u8e2a\u5b83\u4eec\uff0c\u7279\u522b\u662f\u5728\u4f7f\u7528\u677f\uff08plate\uff09\u65f6\u3002\u4f7f\u7528\u00a0FlowJo v10\u00a0\u7684\u677f\u7f16\u8f91\u5668\uff0c\u60a8\u5b9e\u9645\u4e0a\u53ef\u4ee5\u5c06\u8be5\u00a0excel\u00a0\u6587\u4ef6\u4fdd\u5b58\u4e3a\u00a0CSV\u00a0\u5e76\u5c06\u5176\u5bfc\u5165\u677f\u7f16\u8f91\u5668\u3002\u7136\u540e\uff0c\u60a8\u53ef\u4ee5\u5728\u00a0FlowJo\u00a0\u4e2d\u4ece\u5404\u4e2a\u5b54(wells)\u521b\u5efa\u7ec4\uff0c\u5e76\u5c06\u8fd9\u4e9b\u5173\u952e\u5b57\u6dfb\u52a0\u5230\u60a8\u7684\u6570\u636e\u6587\u4ef6\u4e2d\u3002<\/p>\n\n\n\n

\"\"
\u56fe\u00a04\uff1a\u677f\u7f16\u8f91\u5668\u529f\u80fd\u533a<\/strong><\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"

FlowJo\u5de5\u4f5c\u533a\u53ef\u4ee5\u4f7f\u7528\u529f\u80fd\u533a...<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22,4],"tags":[],"class_list":["post-461","post","type-post","status-publish","format-standard","hentry","category-flowjo","category-softapp"],"_links":{"self":[{"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts\/461","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=461"}],"version-history":[{"count":2,"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts\/461\/revisions"}],"predecessor-version":[{"id":543,"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts\/461\/revisions\/543"}],"wp:attachment":[{"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=461"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=461"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=461"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}