{"id":1845,"date":"2026-08-03T15:35:35","date_gmt":"2026-08-03T07:35:35","guid":{"rendered":"https:\/\/cabit.top\/?p=1845"},"modified":"2026-08-03T15:36:13","modified_gmt":"2026-08-03T07:36:13","slug":"complications-fitting-4pl-5pl-models-bioassay-data","status":"publish","type":"post","link":"https:\/\/cabit.top\/?p=1845","title":{"rendered":"\u5bf9\u751f\u7269\u6d4b\u5b9a\u6570\u636e\u62df\u54084PL\u548c5PL\u6a21\u578b\u7684\u590d\u6742\u6027"},"content":{"rendered":"\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"563\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/feature-1.png\" alt=\"\" class=\"wp-image-1846\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/feature-1.png 800w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/feature-1-300x211.png 300w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/feature-1-768x540.png 768w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/feature-1-670x472.png 670w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\u6211\u4eec\u4e4b\u524d\u8ba8\u8bba\u8fc74\u53c2\u6570\u903b\u8f91\uff084PL\uff09\u6a21\u578b\u3002\u6709\u65f6\u60a8\u53ef\u80fd\u4f1a\u770b\u52304PL\u65b9\u7a0b\u7565\u6709\u4e0d\u540c\u7684\u7248\u672c\u2014\u2014\u5b83\u4eec\u5728\u6570\u5b66\u4e0a\u90fd\u662f\u7b49\u4ef7\u7684\u3002\u4f46\u6709\u4e00\u70b9\u9700\u8981\u6ce8\u610f\uff1a\u8fd9\u56db\u4e2a\u53c2\u6570\u7684\u542b\u4e49\u5728\u4e0d\u540c\u7248\u672c\u4e4b\u95f4\u53ef\u80fd\u6709\u6240\u4e0d\u540c\u3002\u5982\u679c\u60a8\u5728\u62df\u54084PL\u6a21\u578b\u65f6\u5f97\u5230\u7684\u53c2\u6570\u503c\u4e0e\u9884\u671f\u76f8\u5dee\u751a\u8fdc\uff0c\u8bf7\u68c0\u67e5\u60a8\u4f7f\u7528\u76844PL\u7248\u672c\u662f\u5426\u4e0e\u60a8\u60f3\u7684\u4e00\u81f4\uff01\u6211\u4eec\u5728<a href=\"https:\/\/www.quantics.co.uk\/blog\/4pl-formula\/\">\u8fd9\u7bc7\u5173\u4e8e4PL\u516c\u5f0f\u7684\u535a\u5ba2<\/a>\u4e2d\u5bf9\u6b64\u8fdb\u884c\u4e86\u66f4\u8be6\u7ec6\u7684\u63a2\u8ba8\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u6b64\uff0c\u6211\u4eec\u5c06\u66f4\u8be6\u7ec6\u5730\u8ba8\u8bba\u8fde\u7eed\u54cd\u5e94\u6570\u636e\u7684\u6a21\u578b\uff0c\u7279\u522b\u662f\u53ef\u80fd\u51fa\u73b0\u7684\u4e00\u4e9b\u95ee\u9898\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">4PL\u662f\u4e00\u6761\u5bf9\u79f0\u66f2\u7ebf\uff0c\u4ece\u4f4e\u5242\u91cf\u5904\u7684\u6e10\u8fd1\u7ebf\uff08\u6052\u5b9a\u503c\uff09\u5f00\u59cb\uff0c\u5448S\u5f62\u4e0a\u5347\uff0c\u6700\u7ec8\u5728\u9ad8\u5242\u91cf\u5904\u5230\u8fbe\u53e6\u4e00\u6761\u6e10\u8fd1\u7ebf\u3002\u4e0b\u56fe\u4e2d\u5c55\u793a\u4e86\u4e00\u4e2a\u751f\u7269\u6d4b\u5b9a\u6570\u636e\u76844PL\u66f2\u7ebf\u793a\u4f8b\u3002A\u548cD\u662f\u6e10\u8fd1\u7ebf\uff0cEC50\u662fC\u7684\u53cd\u5bf9\u6570\u3002B\u662f&#8221;\u659c\u7387\u53c2\u6570&#8221;\uff0c\u4e0e\u659c\u7387\u6210\u6b63\u6bd4\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">4PL\u6a21\u578b\u7684\u65b9\u7a0b\u5f62\u5f0f\u4e3a\uff1a<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">y = D + (A &#8211; D) \/ (1 + (x \/ C)^B)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5176\u4e2dy\u4e3a\u54cd\u5e94\uff0cx\u4e3a\u539f\u59cb\u5242\u91cf\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u5173\u952e\u8981\u70b9<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>4\u53c2\u6570\u903b\u8f91\uff084PL\uff09\u6a21\u578b\u662f\u5bf9\u79f0\u7684\uff0c\u5bf9\u8bb8\u591a\u751f\u7269\u6d4b\u5b9a\u6548\u679c\u826f\u597d\uff0c\u4f46\u5728\u5904\u7406\u4e0d\u5bf9\u79f0\u6570\u636e\u65f6\u5b58\u5728\u56f0\u96be\u30025\u53c2\u6570\u903b\u8f91\uff085PL\uff09\u6a21\u578b\u589e\u52a0\u4e86\u4e00\u4e2a\u989d\u5916\u53c2\u6570\uff08E\uff09\u6765\u5904\u7406\u4e0d\u5bf9\u79f0\u6027\u3002<\/li>\n\n\n\n<li>\u4f7f\u7528\u6700\u5c0f\u4e8c\u4e58\u6cd5\u786e\u5b9a\u6700\u4f73\u62df\u5408\u66f2\u7ebf\uff0c\u4f46\u6709\u65f6\u4f1a\u5bfc\u81f4\u62df\u5408\u6548\u679c\u4e0d\u4f73\uff0c\u7279\u522b\u662f\u5728\u6240\u9009\u6a21\u578b\u4e0e\u6570\u636e\u7279\u5f81\u4e0d\u5339\u914d\u7684\u60c5\u51b5\u4e0b\u30024PL\u53ef\u80fd\u65e0\u6cd5\u62df\u5408\u4e0d\u5bf9\u79f0\u6570\u636e\uff0c\u800c\u5373\u4f7f5PL\u5728\u6570\u636e\u6781\u7aef\u4e0d\u5bf9\u79f0\u65f6\u4e5f\u53ef\u80fd\u4e0d\u9002\u7528\u3002<\/li>\n\n\n\n<li>\u62df\u5408\u4f18\u5ea6\u68c0\u9a8c\u6709\u52a9\u4e8e\u68c0\u6d4b\u4e0d\u826f\u62df\u5408\u3002\u6b64\u5916\uff0c\u659c\u7387\u503c\u6781\u9ad8\u6216\u62df\u5408\u4e0d\u6536\u655b\u7b49\u8b66\u793a\u4fe1\u53f7\u4e5f\u8868\u660e\u53ef\u80fd\u5b58\u5728\u6f5c\u5728\u95ee\u9898\u3002\u8c03\u6574\u5242\u91cf\u95f4\u8ddd\u6216\u4f7f\u7528\u5185\u7f6e\u68c0\u67e5\u529f\u80fd\u7684\u8f6f\u4ef6\u6709\u52a9\u4e8e\u9632\u6b62\u8bef\u5bfc\u6027\u7ed3\u679c\u3002<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"710\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig1-4pl-symmetrical.png\" alt=\"\" class=\"wp-image-1847\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig1-4pl-symmetrical.png 800w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig1-4pl-symmetrical-300x266.png 300w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig1-4pl-symmetrical-768x682.png 768w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig1-4pl-symmetrical-670x595.png 670w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\u56fe1\uff1a4PL\u75314\u4e2a\u53c2\u6570\u5b9a\u4e49<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">5\u53c2\u6570\u903b\u8f91\u6a21\u578b<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">4PL\u901a\u5e38\u80fd\u8f83\u597d\u5730\u62df\u5408\u751f\u7269\u6d4b\u5b9a\u6570\u636e\u3002\u4f46\u7531\u4e8e\u5b83\u662f\u5bf9\u79f0\u7684\uff0c\u56e0\u6b64\u65e0\u6cd5\u5f88\u597d\u5730\u62df\u5408\u4e0d\u5bf9\u79f0\u6570\u636e\u3002\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u5e38\u7528\u7684\u66ff\u4ee3\u65b9\u6848\u662f5\u53c2\u6570\u903b\u8f91\uff085PL\uff09\u6a21\u578b\u3002\u5b83\u4e0e4PL\u7c7b\u4f3c\uff0c\u4f46\u589e\u52a0\u4e86\u4e00\u4e2a\u989d\u5916\u53c2\u6570E\uff0c\u4f7f\u5176\u80fd\u591f\u5904\u7406\u4e0d\u5bf9\u79f0\u6027\u30025PL\u7684\u65b9\u7a0b\uff0c\u5bf9\u4e8e\u54cd\u5e94y\u4e3a\uff1a<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">y = D + (A &#8211; D) \/ (1 + (x \/ C)^B)^E<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e0e4PL\u4e00\u6837\uff0c5PL\u4ece\u4f4e\u5242\u91cf\u5904\u7684\u4e00\u6761\u6e10\u8fd1\u7ebf\u5f00\u59cb\uff0c\u5230\u9ad8\u5242\u91cf\u5904\u7684\u53e6\u4e00\u6761\u6e10\u8fd1\u7ebf\u7ed3\u675f\u3002\u7136\u800c\uff0c\u4ece\u4e00\u6761\u6e10\u8fd1\u7ebf\u5230\u53e6\u4e00\u6761\u6e10\u8fd1\u7ebf\u7684\u66f2\u7ebf\u4e0d\u662f\u5bf9\u79f0\u7684\u3002\u4ee5\u4e0b\u662f\u4e00\u4e2a\u793a\u4f8b\uff1a<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u6e10\u8fd1\u7ebf\u4ecd\u7136\u662fA\u548cD\uff0cB\u4e5f\u4ecd\u7136\u662f\u659c\u7387\u53c2\u6570\u3002\u7136\u800c\uff0c\u57285PL\u4e2d\uff0cEC50\u4e0d\u662fC\u7684\u53cd\u5bf9\u6570\u3002\u5b83\u7684\u8ba1\u7b97\u516c\u5f0f\u4e3a\uff1a<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">EC50 = C \u00d7 (2^(1\/E) &#8211; 1)^(1\/B)<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"720\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig2-5pl-asymmetrical.png\" alt=\"\" class=\"wp-image-1848\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig2-5pl-asymmetrical.png 800w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig2-5pl-asymmetrical-300x270.png 300w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig2-5pl-asymmetrical-768x691.png 768w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig2-5pl-asymmetrical-670x603.png 670w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\u56fe2\uff1a5PL\u589e\u52a0\u4e86\u4e00\u4e2a\u989d\u5916\u53c2\u6570E\uff0c\u7528\u4e8e\u63a7\u5236\u66f2\u7ebf\u7684\u4e0d\u5bf9\u79f0\u6027<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u65b0\u53c2\u6570E\u63a7\u5236\u7740\u4e0d\u5bf9\u79f0\u6027\uff1a\u5982\u679cE\u7b49\u4e8e1\uff0c\u66f2\u7ebf\u662f\u5bf9\u79f0\u7684\u3002\u5bf9\u4e8e\u8f83\u5927\u7684\u503c\uff0c\u9760\u8fd1A\u6e10\u8fd1\u7ebf\u7684\u66f2\u7ebf\u90e8\u5206\u53d8\u5f97\u66f4\u9661\u5ced\u5f2f\u66f2\uff0c\u800c\u9760\u8fd1D\u6e10\u8fd1\u7ebf\u7684\u90e8\u5206\u5219\u8f83\u5e73\u7f13\uff1b\u5f53E\u5c0f\u4e8e1\u65f6\uff0c\u60c5\u51b5\u5219\u76f8\u53cd\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u62df\u5408\u8fc7\u7a0b<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u8981\u7406\u89e3\u4f7f\u75284PL\u548c5PL\u65f6\u53ef\u80fd\u51fa\u73b0\u7684\u4e00\u4e9b\u95ee\u9898\uff0c\u6709\u5fc5\u8981\u4e86\u89e3\u751f\u7269\u6d4b\u5b9a\u7edf\u8ba1\u8f6f\u4ef6\u5728&#8221;\u62df\u5408&#8221;\u6a21\u578b\u65f6\u505a\u4e86\u4ec0\u4e48\u3002\u7edf\u8ba1\u8f6f\u4ef6\u4f7f\u7528\u4e00\u79cd\u79f0\u4e3a&#8221;\u6700\u5c0f\u4e8c\u4e58\u6cd5&#8221;\u7684\u65b9\u6cd5\u8fdb\u884c\u62df\u5408\u3002\u8be5\u65b9\u6cd5\u7684\u4f18\u70b9\u662f\u6570\u5b66\u4e0a\u7b80\u5355\uff0c\u5e76\u4e14\u4e0e\u6570\u636e\u670d\u4ece\u6b63\u6001\u5206\u5e03\u7684\u5047\u8bbe\u4e00\u81f4\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u7a33\u5065\u56de\u5f52\u662f\u53e6\u4e00\u79cd\u66ff\u4ee3\u65b9\u6cd5\uff0c\u5c06\u5728\u540e\u7eed\u7684\u535a\u5ba2\u4e2d\u8ba8\u8bba\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u987e\u540d\u601d\u4e49\uff0c\u6700\u5c0f\u4e8c\u4e58\u6cd5\u62df\u5408\u8bd5\u56fe\u6700\u5c0f\u5316&#8221;\u5e73\u65b9\u548c&#8221;\u2014\u2014\u5373\u6570\u636e\u70b9\u4e0e\u6a21\u578b\u66f2\u7ebf\u4e4b\u95f4\u8ddd\u79bb\u7684\u5e73\u65b9\u548c\u3002\u8fd9\u662f\u5408\u7406\u7684\uff0c\u56e0\u4e3a\u4f7f\u8fd9\u4e9b\u8ddd\u79bb\u53d8\u5c0f\u5c06\u4f7f\u66f2\u7ebf\u66f4\u63a5\u8fd1\u6570\u636e\uff0c\u8fd9\u6b63\u662f\u6211\u4eec\u60f3\u8981\u7684\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u65e0\u6cd5\u901a\u8fc7\u5355\u4e00\u65b9\u7a0b\u76f4\u63a5\u8ba1\u7b97\u51fa4PL\u62165PL\u6a21\u578b\u7684\u6700\u4f73\u62df\u5408\u3002\u76f8\u53cd\uff0c\u751f\u7269\u6d4b\u5b9a\u7edf\u8ba1\u8f6f\u4ef6\u9996\u5148\u5bf9A\u3001B\u3001C\u548cD\uff08\u5982\u679c\u662f5PL\u8fd8\u5305\u62ecE\uff09\u7684\u503c\u8fdb\u884c\u7c97\u7565\u731c\u6d4b\uff0c\u8fd9\u4e9b\u521d\u59cb\u503c\u53ef\u80fd\u79bb\u6700\u4f73\u62df\u5408\u76f8\u53bb\u751a\u8fdc\u3002\u7136\u540e\u5b83\u5c1d\u8bd5\u5bf9\u62df\u5408\u8fdb\u884c\u5fae\u8c03\uff0c\u5e76\u68c0\u67e5\u5e73\u65b9\u548c\u662f\u5426\u51cf\u5c0f\u4e86\u3002\u5982\u679c\u51cf\u5c0f\u4e86\uff0c\u5b83\u63a5\u53d7\u8be5\u53d8\u5316\u5e76\u5c1d\u8bd5\u53e6\u4e00\u4e2a\u8c03\u6574\uff1b\u5982\u679c\u6ca1\u6709\uff0c\u5b83\u5c1d\u8bd5\u5bf9\u66f2\u7ebf\u8fdb\u884c\u4e0d\u540c\u7684\u8c03\u6574\u3002\u8fd9\u4e00\u8fc7\u7a0b\u6301\u7eed\u8fdb\u884c\uff0c\u76f4\u5230\u65e0\u6cd5\u627e\u5230\u80fd\u4f7f\u5e73\u65b9\u548c\u51cf\u5c0f\u7684\u53d8\u5316\uff0c\u6b64\u65f6\u8f6f\u4ef6\u5f97\u51fa\u7ed3\u8bba\uff1a\u5df2\u627e\u5230\u6700\u4f73\u62df\u5408\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u62df\u5408\u4e2d\u7684\u95ee\u9898<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u901a\u5e38\u60c5\u51b5\u4e0b\uff0c\u62df\u5408\u8fc7\u7a0b\u6548\u679c\u826f\u597d\u3002\u7136\u800c\uff0c\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\u5b83\u53ef\u80fd\u4f1a\u7ed9\u51fa\u610f\u60f3\u4e0d\u5230\u7684\u7ed3\u679c\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e00\u4e2a\u4f8b\u5b50\u662f\uff0c\u8f6f\u4ef6\u786e\u5b9a\u7684&#8221;\u6700\u4f73&#8221;\u62df\u5408\u4ecd\u7136\u53ef\u80fd\u975e\u5e38\u7cdf\u7cd5\u3002\u5f53\u6211\u4eec\u8bd5\u56fe\u5c06\u751f\u7269\u6d4b\u5b9a\u6570\u636e\u62df\u5408\u5230\u4e0d\u9002\u5f53\u7684\u6a21\u578b\u65f6\u5c31\u4f1a\u53d1\u751f\u8fd9\u79cd\u60c5\u51b5\u3002\u4f8b\u5982\uff0c\u5982\u679c\u6570\u636e\u662f\u4e0d\u5bf9\u79f0\u7684\uff0c\u4f46\u6211\u4eec\u8bd5\u56fe\u62df\u54084PL\uff0c\u90a3\u4e48\u62df\u5408\u4e0d\u53ef\u80fd\u5728\u6240\u6709\u5730\u65b9\u90fd\u63a5\u8fd1\u6570\u636e\uff0c\u56e0\u4e3a4PL\u672c\u8d28\u4e0a\u662f\u662f\u5bf9\u79f0\u7684\u3002\u53e6\u4e00\u4e2a\u4f8b\u5b50\u662f\uff0c\u5982\u679c\u751f\u7269\u6d4b\u5b9a\u6570\u636e\u6781\u7aef\u4e0d\u5bf9\u79f0\uff0c\u800c\u6211\u4eec\u8bd5\u56fe\u62df\u54085PL\u3002\u867d\u71365PL\u53ef\u4ee5\u5904\u7406\u4e0d\u5bf9\u79f0\u6027\uff0c\u4f46\u5b83\u80fd\u5904\u7406\u7684\u4e0d\u5bf9\u79f0\u6027\u6709\u4e00\u4e2a\u6700\u5927\u8303\u56f4\u3002\u56fe3\u5c55\u793a\u4e865PL\u6a21\u578b\u53ef\u80fd\u5b9e\u73b0\u7684\u4e0d\u5bf9\u79f0\u6027\u7684\u5b8c\u6574\u8303\u56f4\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"716\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig3-max-asymmetry-1024x716.png\" alt=\"\" class=\"wp-image-1849\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig3-max-asymmetry-1024x716.png 1024w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig3-max-asymmetry-300x210.png 300w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig3-max-asymmetry-768x537.png 768w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig3-max-asymmetry-1536x1074.png 1536w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig3-max-asymmetry-2048x1433.png 2048w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig3-max-asymmetry-670x469.png 670w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\u56fe3\uff1a5PL\u6a21\u578b\u62df\u5408\u53ef\u80fd\u5b9e\u73b0\u7684\u4e0d\u5bf9\u79f0\u6027\u7684\u5b8c\u6574\u8303\u56f4<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e0a\u56fe\u4e2d\u7684\u6240\u6709\u66f2\u7ebf\u90fd\u662f5PL\uff0c\u5177\u6709\u4e0d\u540c\u7684E\u53c2\u6570\u503c\uff08\u5141\u8bb8\u4e0d\u5bf9\u79f0\u7684\u989d\u5916\u53c2\u6570\uff09\u3002\u9ed1\u8272\u66f2\u7ebf\u662fE=1\uff0c\u6b64\u65f65PL\u662f\u5bf9\u79f0\u7684\uff0c\u865a\u7ebf\u66f2\u7ebf\u662fE\u7684\u5176\u4ed6\u503c\u3002\u7136\u800c\uff0c\u4e0d\u53ef\u80fd\u8d85\u8d8a\u53f3\u4fa7\u7684\u7d2b\u8272\u5b9e\u7ebf\u66f2\u7ebf\uff08E=0\uff09\u6216\u5de6\u4fa7\u7684\u7ea2\u8272\u5b9e\u7ebf\u66f2\u7ebf\uff08E=\u221e\uff09\u3002\u56e0\u6b64\uff0c\u5982\u679c\u60a8\u7684\u751f\u7269\u6d4b\u5b9a\u6570\u636e\u6bd4\u8fd9\u66f4\u52a0\u4e0d\u5bf9\u79f0\uff0c\u5219\u65e0\u6cd5\u75285PL\u83b7\u5f97\u826f\u597d\u7684\u62df\u5408\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u9002\u5f53\u7684\u62df\u5408\u4f18\u5ea6\u68c0\u9a8c\uff0c\u4f8b\u5982F\u68c0\u9a8c\uff0c\u6709\u52a9\u4e8e\u68c0\u67e5\u662f\u5426\u53d1\u751f\u4e86\u8fd9\u79cd\u60c5\u51b5\u3002\u53ea\u8981\u6709\u4e24\u4e2a\u6216\u66f4\u591a\u91cd\u590d\uff0cF\u68c0\u9a8c\u5c31\u53ef\u4ee5\u7528\u4f5c\u4efb\u4f55\u6a21\u578b\u7684\u62df\u5408\u4f18\u5ea6\u68c0\u9a8c\u3002F\u68c0\u9a8c\u6bd4\u8f83\u4e24\u4e2a\u91cf\uff1a&#8221;\u5931\u62df\u8bef\u5dee&#8221;\uff0c\u7528\u4e8e\u5ea6\u91cf\u62df\u5408\u4e0e\u751f\u7269\u6d4b\u5b9a\u6570\u636e\u4e4b\u95f4\u7684\u8ddd\u79bb\uff1b\u4ee5\u53ca&#8221;\u7eaf\u8bef\u5dee&#8221;\uff0c\u7528\u4e8e\u5ea6\u91cf\u91cd\u590d\u4e4b\u95f4\u7684\u5e73\u5747\u5dee\u5f02\u3002\u5982\u679c\u524d\u8005\u76f8\u6bd4\u540e\u8005\u8f83\u5927\uff0c\u5219F\u68c0\u9a8c\u7684p\u503c\u5c06\u8f83\u5c0f\uff0c\u8868\u660e\u62df\u5408\u6548\u679c\u4e0d\u4f73\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u63a8\u8350\u9605\u8bfb\u2026<\/strong><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/www.quantics.co.uk\/blog\/4pl-formula\/\">\u4ec0\u4e48\u662f4PL\u516c\u5f0f\uff1f<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/www.quantics.co.uk\/blog\/5pl-formula\/\">\u4ec0\u4e48\u662f5PL\u516c\u5f0f\uff1f<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/www.quantics.co.uk\/blog\/r\u00b2-ii-what-should-you-use-to-measure-goodness-of-fit\/\">\u6211\u4eec\u63a5\u8fd1R\u00b2\u4e86\u5417\uff1f\u4e3a\u4ec0\u4e48R\u00b2\u662f\u62df\u5408\u4f18\u5ea6\u7684\u4e0d\u826f\u6307\u6807<\/a><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u53e6\u4e00\u4e2a\u95ee\u9898\u662f\uff0c\u62df\u5408\u53ef\u80fd\u63a5\u8fd1\u6570\u636e\uff0c\u4f46\u6a21\u578b\u66f2\u7ebf\u53ef\u80fd\u4e0d\u7b26\u5408\u9884\u671f\u3002\u5bf9\u4e8e4PL\uff0c\u6211\u4eec\u53ef\u80fd\u4f1a\u5f97\u5230\u50cf\u56fe4\u8fd9\u6837\u7684\u66f2\u7ebf\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"713\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig4-infinite-b-1024x713.png\" alt=\"\" class=\"wp-image-1850\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig4-infinite-b-1024x713.png 1024w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig4-infinite-b-300x209.png 300w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig4-infinite-b-768x535.png 768w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig4-infinite-b-670x466.png 670w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/08\/fig4-infinite-b.png 1250w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\u56fe4\uff1a\u4e00\u4e2a\u5931\u8d25\u76844PL\u6a21\u578b\u62df\u5408<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u8fd9\u662f\u600e\u4e48\u56de\u4e8b\u5462\uff1f<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5bf9\u4e8e\u4e0a\u9762\u6240\u793a\u7684\u751f\u7269\u6d4b\u5b9a\u6570\u636e\uff0c\u6070\u597d\u51fa\u73b0\u4e86\u4e00\u4e2a\u60c5\u51b5\uff1a\u5f53B\u589e\u5927\u65f6\uff0c\u5e73\u65b9\u548c\u603b\u662f\u53d8\u5f97\u66f4\u5c0f\u2014\u2014\u4f46\u968f\u7740B\u7684\u589e\u5927\uff0c\u53d8\u5316\u8d8a\u6765\u8d8a\u7f13\u6162\u3002\u4ece\u6570\u5b66\u4e0a\u8bb2\uff0c\u6700\u4f73\u62df\u5408\uff08\u5b9e\u7ebf\uff09\u5177\u6709\u65e0\u7a77\u5927\u7684B\uff0c\u8fd9\u610f\u5473\u7740\u6a21\u578b\u66f2\u7ebf\u5728\u5230\u8fbeEC50\u4e4b\u524d\u4e00\u76f4\u662f\u5e73\u5766\u7684\uff0c\u7136\u540e\u5728EC50\u5904\u7a81\u7136\u8df3\u53d8\u5230\u53e6\u4e00\u6761\u6e10\u8fd1\u7ebf\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u8fd9\u79cd\u60c5\u51b5\u7684\u4e00\u4e2a\u7279\u6b8a\u95ee\u9898\u662f\uff0c\u5b83\u53ef\u80fd\u4e0d\u50cf\u4e0a\u9762\u7684\u4f8b\u5b50\u90a3\u4e48\u660e\u663e\u3002\u5927\u591a\u6570\u751f\u7269\u6d4b\u5b9a\u7edf\u8ba1\u8f6f\u4ef6\u4f1a\u591a\u6b21\u8c03\u6574\u62df\u5408\uff0c\u4e0d\u65ad\u589e\u52a0B\u7684\u503c\uff0c\u4f46\u6700\u7ec8\u5e73\u65b9\u548c\u7684\u53d8\u5316\u53d8\u5f97\u975e\u5e38\u5c0f\uff0c\u4ee5\u81f3\u4e8e\u8f6f\u4ef6\u8ba4\u4e3a\u5df2\u7ecf\u8fbe\u5230\u6700\u4f73\u62df\u5408\uff0c\u7136\u540e\u505c\u6b62\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u7136\u540e\u5b83\u4f1a\u8f93\u51fa\u5b83\u5df2\u8fbe\u5230\u7684\u4efb\u4f55B\u503c\uff0c\u53ef\u80fd\u4e3a10\u621620\u3002\u8fd9\u5c06\u4ea7\u751f\u9661\u5ced\u7684\u6a21\u578b\u66f2\u7ebf\uff0c\u5982\u56fe\u4e2d\u7684\u865a\u7ebf\u66f2\u7ebf\u6240\u793a\uff0c\u4f46\u6ca1\u6709\u7a81\u7136\u7684\u8df3\u53d8\u3002\u53ef\u80fd\u6ca1\u6709\u4efb\u4f55\u660e\u663e\u7684\u8ff9\u8c61\u8868\u660e\u51fa\u4e86\u95ee\u9898\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e3a\u907f\u514d\u8fd9\u4e2a\u95ee\u9898\uff0c\u8bf7\u52a1\u5fc5\u68c0\u67e5\u8f6f\u4ef6\u8f93\u51fa\u4e2d\u7684\u8b66\u544a\u4fe1\u606f\u2014\u2014\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u8f6f\u4ef6\u8f93\u51fa\u53ef\u80fd\u4f1a\u663e\u793a\u7c7b\u4f3c&#8221;\u62df\u5408\u672a\u6536\u655b&#8221;\u7684\u4fe1\u606f\u2014\u2014\u5e76\u8003\u8651\u4f7f\u7528\u9002\u7528\u6027\u6807\u51c6\u6765\u62d2\u7eddB\u503c\u975e\u5e38\u5927\u7684\u62df\u5408\u3002\u5982\u679c\u8fd9\u79cd\u60c5\u51b5\u53cd\u590d\u53d1\u751f\uff0c\u53ef\u80fd\u9700\u8981\u51cf\u5c0f\u5242\u91cf\u95f4\u8ddd\uff0c\u4ee5\u5728\u66f2\u7ebf\u7684\u9661\u5ced\u90e8\u5206\u83b7\u5f97\u66f4\u591a\u6570\u636e\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Quantics\u7684\u751f\u7269\u6d4b\u5b9a\u8f6f\u4ef6QuBAS\u4f1a\u81ea\u52a8\u5904\u7406\u8fd9\u4e2a\u95ee\u9898\uff1a\u5b83\u4f1a\u4e13\u95e8\u68c0\u67e5\u770b\u4f3c\u6700\u4f73\u7684\u62df\u5408\u662f\u5426\u662f\u4e00\u6761\u5408\u7406\u7684\u5e73\u6ed1\u66f2\u7ebf\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u601d\u8003\uff1a<\/strong>&nbsp;\u4e0a\u9762\u5c55\u793a\u7684\u751f\u7269\u6570\u636e\u771f\u7684\u603b\u662f\u51fa\u4e4e\u610f\u6599\u5417\uff1fGreulich\u7b49\u4eba\u5728<a href=\"https:\/\/doi.org\/10.1016\/j.bpj.2015.04.003\">\u8fd9\u7bc7\u6709\u8da3\u7684\u8bba\u6587<\/a>\u4e2d\u5f15\u53d1\u4e86\u6211\u4eecQuantics\u56e2\u961f\u7684\u601d\u8003\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u56fe4\u4e2d4PL\u6240\u663e\u793a\u7684\u7c7b\u4f3c&#8221;\u8df3\u53d8&#8221;\u4e5f\u53ef\u80fd\u53d1\u751f\u57285PL\u62df\u5408\u4e2d\u3002\u56fe3\u4e2d5PL\u7684\u7d2b\u8272\u66f2\u7ebf\u5c55\u793a\u4e86\u53e6\u4e00\u4e2a\u4e0d\u540c\u7684\u4f8b\u5b50\uff0c\u5176\u4e2d\u54cd\u5e94\u5728\u8fbe\u5230\u67d0\u4e2a\u5242\u91cf\u4e4b\u524d\u5b8c\u5168\u5e73\u5766\uff0c\u7136\u540e\u5728\u8fbe\u5230\u8be5\u5242\u91cf\u65f6\u7a81\u7136\u5f00\u59cb\u6025\u5267\u4e0a\u5347\u3002<\/p>\n\n\n\n<p 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