{"id":1820,"date":"2026-05-31T16:39:53","date_gmt":"2026-05-31T08:39:53","guid":{"rendered":"https:\/\/cabit.top\/?p=1820"},"modified":"2026-05-31T16:40:24","modified_gmt":"2026-05-31T08:40:24","slug":"essentials-bioassay-design-and-relative-potency-determination","status":"publish","type":"post","link":"https:\/\/cabit.top\/?p=1820","title":{"rendered":"\u751f\u7269\u6d4b\u5b9a\u8bbe\u8ba1\u4e0e\u76f8\u5bf9\u6548\u4ef7\u6d4b\u5b9a\u7684\u8981\u70b9"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><em>BioPharm International<\/em>\u00a0\u00a0\u00b7\u00a0 \u7b2c 29 \u5377\uff0c\u7b2c 4 \u671f \u00a0\u00b7\u00a0 2016 \u5e74 \u00a0\u00b7\u00a0 \u9875\u7801 49\u201352 \u00a0\u00b7\u00a0 Thomas A. Little \u535a\u58eb<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u5b9a\u4e49\u751f\u7269\u6d4b\u5b9a\u548c\u76f8\u5bf9\u6548\u4ef7\u65f6\uff0c\u7f8e\u56fd\u836f\u5178\uff08USP\uff09\u6307\u51fa<sup>[1]<\/sup>\uff1a<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p class=\"wp-block-paragraph\">&#8220;\u7531\u4e8e\u751f\u7269\u6d4b\u8bd5\u7cfb\u7edf\uff08\u5305\u62ec\u52a8\u7269\u3001\u7ec6\u80de\u3001\u4eea\u5668\u3001\u8bd5\u5242\u4ee5\u53ca\u65e5\u5e38\u548c\u5b9e\u9a8c\u5ba4\u95f4\u7684\u5dee\u5f02\uff09\u56fa\u6709\u7684\u53d8\u5f02\u6027\uff0c\u6548\u4ef7\u7684\u7edd\u5bf9\u6d4b\u91cf\u6bd4\u76f8\u5bf9\u4e8e\u6807\u51c6\u54c1\u7684\u6d3b\u6027\u6d4b\u91cf\u53d8\u5f02\u66f4\u5927\u3002\u8fd9\u5bfc\u81f4\u4e86\u76f8\u5bf9\u6548\u4ef7\u65b9\u6cd5\u7684\u91c7\u7528\u3002\u5047\u8bbe\u6807\u51c6\u54c1\u548c\u6d4b\u8bd5\u6750\u6599\u5728\u751f\u7269\u5b66\u4e0a\u76f8\u4f3c\uff0c\u5219\u5e94\u5b58\u5728\u7edf\u8ba1\u76f8\u4f3c\u6027\uff08\u8fd9\u662f\u6d4b\u8bd5\u54c1\u4e0e\u6807\u51c6\u54c1\u76f8\u4f3c\u6027\u7684\u7ed3\u679c\uff09\uff0c\u5e76\u4e14\u6d4b\u8bd5\u6837\u54c1\u53ef\u4ee5\u9884\u671f\u8868\u73b0\u4e3a\u6807\u51c6\u54c1\u7684\u6d53\u7f29\u6216\u7a00\u91ca\u3002\u76f8\u5bf9\u6548\u4ef7\u662f\u901a\u8fc7\u6bd4\u8f83\u6d4b\u8bd5\u54c1\u548c\u6807\u51c6\u54c1\u5236\u5242\u7684\u5242\u91cf-\u54cd\u5e94\u5173\u7cfb\u800c\u83b7\u5f97\u7684\u65e0\u91cf\u7eb2\u5ea6\u91cf\u3002&#8221;<\/p>\n<\/blockquote>\n\n\n\n<p class=\"wp-block-paragraph\">\u672c\u6587\u65e8\u5728\u4ecb\u7ecd\u76f8\u5bf9\u6548\u4ef7\u751f\u7269\u6d4b\u5b9a\u4e2d\u6700\u5e38\u89c1\u7684\u7ec4\u6210\u90e8\u5206\uff0c\u5e76\u4e3a\u6d4b\u5b9a\u5f00\u53d1\u3001\u8ba1\u7b97\u548c\u63a7\u5236\u63d0\u4f9b\u4e00\u4e2a\u6846\u67b6\u6216\u5e73\u53f0\u3002\u5b83\u5e76\u975e\u610f\u5728\u6db5\u76d6\u4e0e\u751f\u7269\u6d4b\u5b9a\u76f8\u5173\u7684\u6240\u6709\u95ee\u9898\uff0c\u800c\u662f\u6db5\u76d6\u76f8\u5bf9\u6548\u4ef7\u8ba1\u7b97\u548c\u62a5\u544a\u4e2d\u7684\u57fa\u672c\u7ec4\u6210\u90e8\u5206\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u751f\u7269\u6d4b\u5b9a\u7684\u76ee\u7684\u4e0e\u9002\u7528\u6027<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u751f\u7269\u6d4b\u5b9a\u53ef\u4ee5\u662f\u4f53\u5916\u3001\u4f53\u5185\u6216\u79bb\u4f53\u5b9e\u9a8c\uff0c\u53ef\u7528\u4e8e\u591a\u79cd\u76ee\u7684\uff0c\u5305\u62ec\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5de5\u827a\u5f00\u53d1\u3001\u5de5\u827a\u8868\u5f81\u548c\u4ea7\u54c1\u5f00\u53d1<\/li>\n\n\n\n<li>\u539f\u6599\u836f\u6216\u5236\u5242\u7684\u4ea7\u54c1\u653e\u884c\u6d4b\u8bd5<\/li>\n\n\n\n<li>\u8fc7\u7a0b\u63a7\u5236\u548c\u4e2d\u95f4\u4f53\u6d4b\u8bd5<\/li>\n\n\n\n<li>\u7a33\u5b9a\u6027\u548c\u4ea7\u54c1\u5b8c\u6574\u6027\u6d4b\u8bd5<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">\u751f\u7269\u6d4b\u5b9a\u5242\u91cf\u54cd\u5e94<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u6b63\u786e\u786e\u5b9a\u751f\u7269\u6d4b\u5b9a\u5242\u91cf\u54cd\u5e94\u7a00\u91ca\u65b9\u6848\u662f\u751f\u7269\u6d4b\u5b9a\u6700\u91cd\u8981\u7684\u65b9\u9762\u4e4b\u4e00\u3002\u53ef\u4ee5\u4f7f\u7528\u591a\u79cd\u5242\u91cf\u54cd\u5e94\u7b56\u7565\uff0c\u4f8b\u5982\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5355\u5242\u91cf\uff08\u4e0d\u63a8\u8350\uff09<\/li>\n\n\n\n<li>\u53cc\u5242\u91cf\uff08\u4e0d\u63a8\u8350\uff09<\/li>\n\n\n\n<li>\u4e09\u5242\u91cf\uff08\u4e0d\u63a8\u8350\uff09<\/li>\n\n\n\n<li>\u56db\u5242\u91cf\uff08\u826f\u597d\uff0c\u4f46\u96be\u4ee5\u63a9\u853d\u9971\u548c\u5242\u91cf\uff09<\/li>\n\n\n\n<li>\u4e94\u5242\u91cf\uff08\u9002\u7528\u4e8e\u5e73\u884c\u7ebf\u5206\u6790 [PLA] \u548c\u5242\u91cf\u63a9\u853d\uff09<\/li>\n\n\n\n<li>\u7cbe\u7ec6\u5242\u91cf\uff08\u89c1\u56fe 1\uff09\u6216\u66f4\u591a\uff08\u9002\u7528\u4e8e\u56db\u53c2\u6570 [4PL] \u62df\u5408\u548c\u5242\u91cf\u63a9\u853d\uff09<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e00\u822c\u6765\u8bf4\uff0c\u5e94\u907f\u514d\u4f7f\u7528\u4e00\u3001\u4e8c\u3001\u4e09\u5242\u91cf\u65b9\u6848\uff0c\u56e0\u4e3a\u5b83\u4eec\u96be\u4ee5\u63a7\u5236\u53d8\u5f02\uff0c\u5e76\u4e14\u4f1a\u5728\u76f8\u5bf9\u6548\u4ef7\u8ba1\u7b97\u4e2d\u5e26\u6765\u95ee\u9898\u3002\u7406\u60f3\u7684\u5242\u91cf\u54cd\u5e94\u66f2\u7ebf\u662f\u5e73\u884c\u7ebf\u5206\u6790\uff08\u7ebf\u6027\uff09\u7684\u4e94\u70b9\u66f2\u7ebf\uff0c\u4ee5\u53ca S \u5f62\u62df\u5408\u7684\u4e5d\u70b9\u6216\u66f4\u591a\u5242\u91cf\u65b9\u6848\u3002\u5bf9\u4e8e S \u5f62\u66f2\u7ebf\uff0c\u7406\u60f3\u60c5\u51b5\u4e0b\uff0c\u5728\u66f2\u7ebf\u7684\u975e\u6d3b\u6027\u6216\u4f4e\u54cd\u5e94\u90e8\u5206\u5e94\u6709\u4e09\u4e2a\u70b9\uff0c\u5728\u7ebf\u6027\u90e8\u5206\u6709\u4e09\u4e2a\u70b9\uff0c\u5728\u4ece\u7cfb\u5217\u7a00\u91ca\u7684\u5bf9\u6570\u6216 log<sub>10<\/sub>&nbsp;\u5c3a\u5ea6\u7684\u9971\u548c\u533a\u57df\u6709\u4e09\u4e2a\u70b9\u3002\u5728\u5e73\u884c\u7ebf\u5206\u6790\uff08\u975e S \u5f62\uff09\u4e2d\uff0c\u4e94\u5242\u91cf\u65b9\u6848\u5728\u66f2\u7ebf\u7684\u7ebf\u6027\u90e8\u5206\u662f\u7406\u60f3\u7684\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"495\" height=\"355\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-32.png\" alt=\"\" class=\"wp-image-1821\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-32.png 495w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-32-300x215.png 300w\" sizes=\"auto, (max-width: 495px) 100vw, 495px\" \/><figcaption class=\"wp-element-caption\"><strong>\u56fe 1\uff1a<\/strong>\u7cbe\u7ec6\u5242\u91cf\u65b9\u6848\u793a\u610f\u56fe \u2014 \u5728 S \u5f62\u66f2\u7ebf\u7684\u975e\u6d3b\u6027\u533a\u3001\u7ebf\u6027\u533a\u548c\u9971\u548c\u533a\u5404\u8bbe\u7f6e\u4e09\u4e2a\u70b9\u3002<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">4PL \u4e0e 5PL \u62df\u5408<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u5982\u679c\u62df\u5408 logistic S \u5f62\u66f2\u7ebf\uff0c\u6709\u4e24\u79cd\u5e38\u89c1\u7684\u66f2\u7ebf\u62df\u5408\u6280\u672f\uff1a\u56db\u53c2\u6570\uff084PL\uff09\u548c\u4e94\u53c2\u6570\uff085PL\uff09\u3002\u56db\u53c2\u6570\u66f2\u7ebf\u5047\u8bbe\u66f2\u7ebf\u62df\u5408\u7684\u4e0a\u90e8\u548c\u4e0b\u90e8\u533a\u57df\u5bf9\u79f0\uff0c\u800c\u4e94\u53c2\u6570\u66f2\u7ebf\u5047\u8bbe\u4e0a\u4e0b\u6e10\u8fd1\u7ebf\u7684\u66f2\u7ebf\u5f62\u72b6\u4e0d\u540c\u3002\u8bc4\u4f30 4PL \u62df\u5408\u7684\u6b8b\u5dee\u53ef\u4ee5\u6307\u793a\u66f2\u7ebf\u62df\u5408\u6280\u672f\u662f\u5426\u5408\u9002\uff0c\u6216\u8005\u662f\u5426\u9700\u8981\u8fdb\u884c 5PL \u62df\u5408\u3002\u5982\u679c 4PL \u62df\u5408\u826f\u597d\uff0c\u6b8b\u5dee\u5e94\u5448\u968f\u673a\u6027\u548c\u6b63\u6001\u6027\uff0c\u5e76\u4e14\u5728\u5242\u91cf\u54cd\u5e94\u8303\u56f4\u5185\u65e0\u7cfb\u7edf\u6a21\u5f0f\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u5f02\u5e38\u503c\u5206\u6790<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u8ba8\u8bba\u5f02\u5e38\u503c\uff08\u89c1\u56fe 2\uff09\u65f6\uff0cUSP &lt;1032&gt;\u6307\u51fa\uff1a&#8221;\u751f\u7269\u6d4b\u5b9a\u5f00\u53d1\u7684\u7edf\u8ba1\u8981\u7d20\u5305\u62ec\u6570\u636e\u7c7b\u578b\u3001\u4e0d\u540c\u6d53\u5ea6\u4e0b\u7684\u54cd\u5e94\u6d4b\u91cf\u3001\u6d4b\u5b9a\u8bbe\u8ba1\u3001\u7edf\u8ba1\u6a21\u578b\u3001\u6570\u636e\u7684\u9884\u5206\u6790\u5904\u7406\u3001\u6570\u636e\u5206\u6790\u65b9\u6cd5\u3001\u9002\u5b9c\u6027\u6d4b\u8bd5\u548c\u5f02\u5e38\u503c\u5206\u6790\u3002\u8fd9\u4e9b\u6784\u6210\u4e86\u5c06\u7528\u4e8e\u4f30\u8ba1\u6d4b\u8bd5\u6837\u54c1\u6548\u4ef7\u7684\u751f\u7269\u6d4b\u5b9a\u7cfb\u7edf\u7684\u7ec4\u6210\u90e8\u5206\u3002&#8221;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u4ee5\u4e0b\u662f\u63a8\u8350\u7684\u5f02\u5e38\u503c\u68c0\u6d4b\u548c\u5254\u9664\u65b9\u6cd5<sup>[2]<\/sup>\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u66f2\u7ebf\u62df\u5408\u7684\u5b66\u751f\u5316\u6b8b\u5dee\u5927\u4e8e 2.575\uff0899% \u98ce\u9669\uff09<\/li>\n\n\n\n<li>\u88ab\u5254\u9664\u70b9\u7684 z \u5206\u6570\u4e3a 3 \u6216\u66f4\u5927\uff0899.73% \u98ce\u9669\uff09<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u88ab\u5254\u9664\u70b9\u5bf9\u66f2\u7ebf\u7684\u5f71\u54cd\u53ef\u901a\u8fc7\u68c0\u67e5 R\u00b2 \u548c\u6b8b\u5dee\u5747\u65b9\u6839\u8bef\u5dee\uff08RMSE\uff09\u7684\u53d8\u5316\u6765\u8bc4\u4f30\u3002\u4e00\u822c\u6765\u8bf4\uff0c\u5728\u5242\u91cf\u66f2\u7ebf\u4e0a\u4e0d\u5e94\u5254\u9664\u8d85\u8fc7\u4e00\u4e2a\u70b9\u6216\u4e00\u4e2a\u5242\u91cf\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"495\" height=\"249\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-33.png\" alt=\"\" class=\"wp-image-1822\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-33.png 495w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-33-300x151.png 300w\" sizes=\"auto, (max-width: 495px) 100vw, 495px\" \/><figcaption class=\"wp-element-caption\"><strong>\u56fe 2\uff1a<\/strong>\u5f02\u5e38\u503c\u5206\u6790\u793a\u4f8b \u2014 \u8bc6\u522b\u5242\u91cf\u54cd\u5e94\u66f2\u7ebf\u4e2d\u504f\u79bb\u7684\u6d4b\u91cf\u70b9\u3002<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u53d8\u6362\u548c\u66f2\u7ebf\u52a0\u6743<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u5982\u679c\u80fd\u591f\u8bc1\u660e\u53d8\u6362\u53ef\u4ee5\u6539\u5584\u6d4b\u5b9a\u7684\u7ebf\u6027\u5ea6\uff0c\u5219\u53ef\u4ee5\u5728\u7a00\u91ca\u548c\u6d4b\u91cf\u54cd\u5e94\u4e24\u65b9\u9762\u90fd\u8fdb\u884c\u6570\u636e\u53d8\u6362\u3002USP &lt;1034&gt;<sup>[3]<\/sup>\u6307\u51fa\uff1a&#8221;\u62df\u5408\u7edf\u8ba1\u6a21\u578b\u4ee5\u68c0\u6d4b\u6f5c\u5728\u5f02\u5e38\u503c\uff08\u5728\u5f00\u53d1\u8fc7\u7a0b\u4e2d\u9009\u5b9a\uff09\uff0c\u5305\u62ec\u4efb\u4f55\u52a0\u6743\u548c\u53d8\u6362\u3002\u9996\u5148\u5728\u4e0d\u5047\u8bbe\u6d4b\u8bd5\u54c1\u548c\u6807\u51c6\u54c1\u66f2\u7ebf\u76f8\u4f3c\u7684\u60c5\u51b5\u4e0b\u8fdb\u884c\uff0c\u4f46\u5e94\u5305\u542b\u8bbe\u8ba1\u7ed3\u6784\u7684\u91cd\u8981\u5143\u7d20\uff0c\u7406\u60f3\u60c5\u51b5\u4e0b\u4f7f\u7528\u6bd4\u7528\u4e8e\u8bc4\u4f30\u76f8\u4f3c\u6027\u7684\u6a21\u578b\u5bf9\u54cd\u5e94\u51fd\u6570\u5f62\u5f0f\u5047\u8bbe\u66f4\u5c11\u7684\u6a21\u578b\u3002&#8221;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5982\u679c\u5242\u91cf\u54cd\u5e94\u8303\u56f4\u5185\u7684\u53d8\u5f02\u7f3a\u4e4f\u540c\u8d28\u6027\uff0c\u52a0\u6743\u53ef\u4ee5\u51cf\u5c11\u76f8\u5bf9\u6548\u4ef7\u6d4b\u5b9a\u7684\u53d8\u5f02\u3002\u901a\u5e38\uff0c\u5f53\u53d8\u5f02\u5728\u7edf\u8ba1\u4e0a\u663e\u8457\u4e0d\u540c\u65f6\uff0c\u4f7f\u7528\u6bcf\u4e2a\u5242\u91cf\u4e0b\u6b8b\u5dee\u7684 1\/\u65b9\u5dee\u3002\u53ef\u4ee5\u4f7f\u7528 Bartlett \u6216 Levene \u7684\u65b9\u5dee\u4e0d\u76f8\u7b49\u68c0\u9a8c\u6765\u786e\u5b9a\u65b9\u5dee\u662f\u5426\u5728\u7edf\u8ba1\u4e0a\u663e\u8457\u4e0d\u540c\u3002\u901a\u5e38\u63a8\u8350\u4f7f\u7528\u56fa\u5b9a\u7684 1\/\u65b9\u5dee\u52a0\u6743\u65b9\u6cd5\u3002\u52a0\u6743\u9002\u7528\u4e8e\u53c2\u6bd4\u54c1\u548c\u6d4b\u8bd5\u54c1\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u7528\u4e8e\u5e73\u884c\u7ebf\u5206\u6790\u7684\u63a9\u853d<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u7ebf\u6027\u5e73\u884c\u7ebf\u5206\u6790\u4e2d\uff0c\u5efa\u8bae\u63a9\u853d\u6216\u5254\u9664\u9971\u548c\u6d4b\u91cf\u503c\uff08\u89c1\u56fe 3\uff09\u3002\u4ec5\u5254\u9664\u54cd\u5e94\u66f2\u7ebf\u7684\u9ad8\u5242\u91cf\u6216\u4f4e\u5242\u91cf\uff08\u9971\u548c\uff09\u90e8\u5206\uff0c\u6839\u636e\u5242\u91cf\u65b9\u6848\u7684\u4e0d\u540c\uff0c\u4f7f\u7528\u8fde\u7eed\u7684\u4e09\u6216\u56db\u4e2a\u5242\u91cf\u8fdb\u884c\u76f8\u5bf9\u6548\u4ef7\u6d4b\u5b9a\u3002\u63a9\u853d\u5c06\u6d88\u9664\u5e73\u884c\u7ebf\u5206\u6790\u4e2d\u7684\u9971\u548c\u6548\u5e94\uff08\u66f2\u68cd\u7403\u68d2\u6548\u5e94\uff09\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"495\" height=\"207\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-34.png\" alt=\"\" class=\"wp-image-1823\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-34.png 495w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-34-300x125.png 300w\" sizes=\"auto, (max-width: 495px) 100vw, 495px\" \/><figcaption class=\"wp-element-caption\"><strong>\u56fe 3\uff1a<\/strong>\u63a9\u853d\u793a\u610f\u56fe \u2014 \u5728\u7ebf\u6027\u5e73\u884c\u7ebf\u5206\u6790\u4e2d\u5254\u9664\u9971\u548c\u5242\u91cf\u70b9\uff08\u9ad8\u5242\u91cf\u6216\u4f4e\u5242\u91cf\uff09\u3002<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u7cfb\u7edf\u9002\u7528\u6027\u548c\u5e73\u884c\u6027\u8bc4\u4f30<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u6709\u591a\u79cd\u6280\u672f\u53ef\u7528\u4e8e\u8bc4\u4f30\u5e73\u884c\u6027\u3002\u8868 I \u63d0\u4f9b\u4e86\u8bc4\u4f30\u5e73\u884c\u6027\u7684\u65b9\u6cd5\u603b\u7ed3\u3002\u5176\u4ed6\u7cfb\u7edf\u9002\u7528\u6027\u6807\u51c6\u5305\u62ec\u5df2\u77e5\u5bf9\u7167\u54c1\u7684\u6d4b\u91cf\u3001\u91cd\u590d\u6d4b\u91cf\u7684\u76f8\u5bf9\u53d8\u5f02\uff08%CV\uff09\u4ee5\u53ca\u7528\u4e8e\u4fe1\u53f7\u5f3a\u5ea6\u7684\u66f2\u7ebf\u6df1\u5ea6\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"495\" height=\"323\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-35.png\" alt=\"\" class=\"wp-image-1824\" style=\"aspect-ratio:1.5325312463497256;width:616px;height:auto\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-35.png 495w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-35-300x196.png 300w\" sizes=\"auto, (max-width: 495px) 100vw, 495px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u8868 I\uff1a<\/strong>\u5e73\u884c\u6027\u548c\u7cfb\u7edf\u9002\u7528\u6027\u8bc4\u4f30\u65b9\u6cd5\u603b\u7ed3\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u76f8\u5bf9\u6548\u4ef7\u7684\u8ba1\u7b97<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u76f8\u5bf9\u6548\u4ef7\u7684\u8ba1\u7b97\u5982\u516c\u5f0f 1 \u6240\u793a\uff0c\u5e76\u53ef\u80fd\u56e0\u6d4b\u5b9a\u800c\u5f02\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u516c\u5f0f 1\uff1a<\/strong>\u00a0\u76f8\u5bf9\u6548\u4ef7 = (EC<sub>50[\u6d4b\u8bd5\u54c1]<\/sub>\u00a0\/ EC<sub>50[\u53c2\u6bd4\u54c1]<\/sub>) \u00d7100 +  CF<sub>\u53c2\u6bd4\u54c1<\/sub>\u00a0+  CF<sub>\u53c2\u6bd4\u54c1\u7a33\u5b9a\u6027<\/sub><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">EC<sub>50<\/sub>&nbsp;\u6839\u636e 4PL \u7ea6\u675f\u62d0\u70b9\u8ba1\u7b97\uff0c\u5e76\u6839\u636e\u7a00\u91ca\u56e0\u5b50\u662f log<sub>10<\/sub>&nbsp;\u8fd8\u662f log \u53d8\u6362\u8fdb\u884c\u53cd\u53d8\u6362\uff08\u53cd\u5bf9\u6570\uff09\uff08\u89c1\u516c\u5f0f 2\uff09\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u516c\u5f0f 2\uff1a<\/strong>log10\u00a0EC<sub>50<\/sub>\u00a0= 10<sup>\u62d0\u70b9<\/sup>\u00a0<br>\u6216 log EC<sub>50<\/sub>\u00a0= Exp(\u62d0\u70b9)<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u63a7\u5236\u6807\u51c6\u54c1\u5bf9\u76f8\u5bf9\u6548\u4ef7\u7684\u5f71\u54cd<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u6ce8\u610f\u5728\u516c\u5f0f 1 \u4e2d\uff0c\u6709\u4e00\u4e2a\u6821\u6b63\u56e0\u5b50\uff08CF\uff09\u5e94\u7528\u4e8e\u76f8\u5bf9\u6548\u4ef7\u6d4b\u5b9a\u3002CF<sub>\u53c2\u6bd4\u54c1<\/sub>&nbsp;\u662f\u5bf9\u65b0\u53c2\u6bd4\u54c1\u8fdb\u884c\u786e\u8ba4\u7684\u6821\u6b63\u56e0\u5b50\uff0c\u7528\u4e8e\u4fee\u6b63\u56e0\u53c2\u6bd4\u54c1\u66f4\u6362\u800c\u5bfc\u81f4\u7684\u6548\u4ef7\u504f\u79fb\u3002CF<sub>\u53c2\u6bd4\u54c1\u7a33\u5b9a\u6027<\/sub>&nbsp;\u662f\u5bf9\u53c2\u6bd4\u54c1\u968f\u65f6\u95f4\u635f\u5931\u6548\u4ef7\u7684\u6821\u6b63\u3002\u672a\u5728\u6548\u4ef7\u8ba1\u7b97\u5668\u4e2d\u5305\u542b\u8fd9\u4e9b\u6821\u6b63\u56e0\u5b50\u5c06\u5bfc\u81f4\u6d4b\u5b9a\u968f\u65f6\u95f4\u63a8\u79fb\u53d8\u5f97\u8fc7\u9ad8\u6216\u8fc7\u4f4e\u4f30\u8ba1\u6548\u4ef7\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u6548\u4ef7\u6d4b\u5b9a\u4e2d\u7684\u5de5\u4f5c\u6d41\u7a0b<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u56fe 4 \u5c55\u793a\u4e86\u751f\u7269\u6d4b\u5b9a\u7684\u826f\u597d\u5de5\u4f5c\u6d41\u7a0b\u3002\u6765\u81ea\u751f\u7269\u6d4b\u5b9a\u7684\u6570\u636e\u5e94\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\u68c0\u67e5\uff0c\u6240\u6709\u53ef\u62a5\u544a\u7ed3\u679c\u5e94\u53d1\u9001\u81f3\u5b9e\u9a8c\u5ba4\u4fe1\u606f\u7ba1\u7406\u7cfb\u7edf\uff08LIMS\uff09\u3002\u751f\u7269\u6d4b\u5b9a\u7684\u5173\u952e\u53c2\u6570\u548c\u8bd5\u5242\u5e94\u7528\u4e8e\u8ddf\u8e2a\u548c\u8d8b\u52bf\u5206\u6790\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"495\" height=\"337\" src=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-36.png\" alt=\"\" class=\"wp-image-1827\" srcset=\"https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-36.png 495w, https:\/\/cabit.top\/wp-content\/uploads\/2026\/05\/image-36-300x204.png 300w\" sizes=\"auto, (max-width: 495px) 100vw, 495px\" \/><figcaption class=\"wp-element-caption\"><strong>\u56fe 4\uff1a<\/strong>\u751f\u7269\u6d4b\u5b9a\u5de5\u4f5c\u6d41\u7a0b \u2014 \u6570\u636e\u91c7\u96c6 \u2192 \u8d28\u91cf\u63a7\u5236\u68c0\u67e5 \u2192 \u5206\u6790 \u2192 LIMS \u62a5\u544a \u2192 \u8ddf\u8e2a\u4e0e\u8d8b\u52bf\u5206\u6790\u3002<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u76d1\u6d4b\u548c\u63a7\u5236\u76f8\u5bf9\u6548\u4ef7<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u957f\u671f\u76d1\u6d4b\u751f\u7269\u6d4b\u5b9a\u7684\u6027\u80fd\u81f3\u5173\u91cd\u8981\u3002\u65e0\u7ea6\u675f\u7684\u6807\u51c6\u54c1 EC<sub>50<\/sub>&nbsp;\u901a\u5e38\u88ab\u8ba4\u4e3a\u662f\u6d4b\u5b9a\u7a33\u5b9a\u6027\u548c\u4e00\u81f4\u6027\u7684\u6700\u4f73\u5ea6\u91cf\u3002\u4e3a\u68c0\u6d4b\u6d4b\u5b9a\u6f02\u79fb\u662f\u65b9\u6cd5\u95ee\u9898\u8fd8\u662f\u6807\u51c6\u54c1\u95ee\u9898\uff0c\u5e94\u4e0e\u65e0\u7ea6\u675f\u7684 EC<sub>50<\/sub>&nbsp;\u8fdb\u884c\u6bd4\u8f83\u3002\u5982\u679c\u53c2\u6bd4\u54c1\u548c\u6d4b\u8bd5\u54c1\u90fd\u5728\u6f02\u79fb\uff0c\u5219\u8868\u660e\u6d4b\u5b9a\u968f\u65f6\u95f4\u53d1\u751f\u4e86\u53d8\u5316\uff0c\u4f46\u5bf9\u76f8\u5bf9\u6548\u4ef7\u6ca1\u6709\u5f71\u54cd\u3002\u5982\u679c\u6d4b\u8bd5\u54c1\u7a33\u5b9a\u800c\u53c2\u6bd4\u54c1\u5448\u8d8b\u52bf\u6027\u53d8\u5316\uff0c\u5219\u4f1a\u5f71\u54cd\u76f8\u5bf9\u6548\u4ef7\uff0c\u5e94\u8fdb\u884c\u7ea0\u6b63\u6216\u9009\u62e9\u65b0\u7684\u53c2\u6bd4\u54c1\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u7ed3\u8bba<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u751f\u7269\u6d4b\u5b9a\u662f\u76f8\u5bf9\u6548\u4ef7\u548c\u751f\u7269\u6d3b\u6027\u7684\u6709\u529b\u6d4b\u8bd5\uff0c\u4f46\u5728\u6d4b\u5b9a\u8bbe\u8ba1\u548c\u5206\u6790\u4e2d\u9700\u8981\u8c28\u614e\uff0c\u4ee5\u786e\u4fdd\u5b83\u4eec\u80fd\u591f\u53ef\u9760\u5730\u6307\u793a\u6548\u4ef7\u7684\u53d8\u5316\u3002\u6d4b\u5b9a\u9a8c\u8bc1<sup>[4]<\/sup>\u5c06\u8bc1\u660e\u6d4b\u5b9a\u7684\u51c6\u786e\u6027\u3001\u91cd\u590d\u6027\u548c\u7ebf\u6027\uff0c\u4ee5\u53ca\u5176\u5728\u6d4b\u91cf\u76f8\u5bf9\u6548\u4ef7\u65b9\u9762\u7684\u9002\u7528\u6027\u3002\u5728\u5242\u91cf\u54cd\u5e94\u9009\u62e9\u3001\u5f02\u5e38\u503c\u68c0\u6d4b\u548c\u5254\u9664\u3001\u63a9\u853d\u3001\u53d8\u6362\u548c\u52a0\u6743\u65b9\u9762\u7684\u8c28\u614e\u9009\u62e9\u5c06\u4f7f\u6d4b\u5b9a\u66f4\u52a0\u7a33\u5b9a\u548c\u53ef\u91cd\u590d\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u53c2\u8003\u6587\u732e<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>USP, &lt;1032> Design and Development of Biological Assay, USP 38\u2013NF 33 (USP, December 2015).<\/li>\n\n\n\n<li>T. Little,\u00a0<em>BioPharm Intern<\/em>\u00a029 (1) (January 2016).<\/li>\n\n\n\n<li>USP, &lt;1034> Analysis of Biological Assays, USP 38\u2013NF 33 (USP, December 2015).<\/li>\n\n\n\n<li>USP, &lt;1033> Biological Assay Validation, USP 38\u2013NF 33 (USP, December 2015).<\/li>\n<\/ol>\n\n\n\n<p class=\"wp-block-paragraph\">\u539f\u6587\u5730\u5740\uff1a<a href=\"https:\/\/www.biopharminternational.com\/view\/essentials-bioassay-design-and-relative-potency-determination\">Essentials in Bioassay Design and Relative Potency Determination | BioPharm International<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>BioPharm Interna&#46;&#46;&#46;<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-1820","post","type-post","status-publish","format-standard","hentry","category-basicknow"],"_links":{"self":[{"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts\/1820","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1820"}],"version-history":[{"count":4,"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts\/1820\/revisions"}],"predecessor-version":[{"id":1829,"href":"https:\/\/cabit.top\/index.php?rest_route=\/wp\/v2\/posts\/1820\/revisions\/1829"}],"wp:attachment":[{"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1820"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1820"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cabit.top\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1820"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}